Naive human pluripotent stem cells

Induced Pluripotent Stem Cells: Challenges and Opportunities

Regenerative capacity of mammals is limited and can rarely regenerate a specific organ or tissue fully. Due to these limitations, regenerative medicine seeks efficient and safe cell sources for regeneration of damaged tissues and organs or treatment for incurable diseases. Human embryonic stem cells (HESCs) hold two important properties called self renewal and pluripotency. However, the use of ...

Naive Pluripotent Stem Cells Derived Directly from Isolated Cells of the Human Inner Cell Mass

Conventional generation of stem cells from human blastocysts produces a developmentally advanced, or primed, stage of pluripotency. In vitro resetting to a more naive phenotype has been reported. However, whether the reset culture conditions of selective kinase inhibition can enable capture of naive epiblast cells directly from the embryo has not been determined. Here, we show that in these spe...

Human-Induced Pluripotent Stem Cells

Human-Induced Pluripotent Stem Cells The ability to reprogram adult mammalian somatic cells to a pluripotent state has proven to be a paradigm shifting discovery. In 2007, this reprogramming technology was translated to the engineering of human-induced pluripotent stem cells (hiPSCs) using skin fibroblasts by both the Yamanaka (transduction of c-Myc, Oct3/4, SOX2, and Klf4) and Thomson (transdu...

Human Testis–Derived Pluripotent Cells and Induced Pluripotent Stem Cells

P-50: Elongating and Elongated Spermatids Manufactured In Vitro from Non-Human Primate Pluripotent Stem Cells

Background: We have recently shown that human embryonic (hESCs) and induced pluripotent stem cells (hiPSCs) can differentiate into advanced spermatogenic cells including round spermatids by in vitro culture (Easley et al., Direct differentiation of human pluripotent stem cells into haploid spermatogenic cells. Cell Reports 2, 440-446 2012) and also, in collaboration, that rhesus spermatogonial ...